Evaluation of the Fluorescence Polarization Assay (FPA) for diagnosis of brucella melitensis infection of goats in Argentina.

Comparison of serological tests for the detection of ovine and caprine antibody to Brucella melitensis.

The indirect enzyme-linked immunosorbent assay (IELISA), the competitive enzyme-linked immunosorbent assay (CELISA) and the fluorescence polarisation assay (FPA) were evaluated with sera from sheep experimentally infected with Brucella melitensis and negative Canadian sheep. The sensitivity and specificity of the assays were as follows: IELISA: 91.7% and 97.6%, CELISA: 75.0% and 99.8% and FPA: ...

Application of the fluorescence polarization assay for detection of caprine antibodies to Brucella melitensis in areas of high prevalence and widespread vaccination.

The screening Rose Bengal test (RBT), the buffered plate agglutination test (BPAT), and the confirmatory complement fixation test (CFT) are currently approved by the World Organization for Animal Health (OIE) for diagnosis of goat brucellosis. However, RBT (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. In the present study, Mexican and Canadian OIE tests were c...

Brucellosis: An Economically Important Infection

Brucellosis is one of the world’s major zoonosis, caused by bacteria of the genus Brucella. The world’s most widespread zoonosis affects cattle, sheep, goats, pigs, and other animals, leading to abortion, infertility, and low milk yields. Humans acquire brucellosis from direct contact with livestock or from drinking unpasteurized milk. Brucella spp. are considered as the most common laboratory-...

Comparison of living vaccines in producing immunity against natural brucella melitensis infection in sheep and goats in Iran

Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats.

Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysacchar...